Hexagonal glycoprotein arrays from Sindbis virus membranes.
نویسندگان
چکیده
Freeze-etch electron microscopy of Sindbis virus and of glycoprotein arrays derived from Sindbis membranes by nonionic detergent treatment shows that the local geometry of glycoprotein-glycoprotein interaction does not depend on the presence of the nucleocapsid.
منابع مشابه
Immunocytochemical study of the partition and distribution of Sindbis virus glycoproteins in freeze-fractured membranes of infected baby hamster kidney cells
Sindbis virus-infected baby hamster kidney cells were analyzed by thin section fracture-label. Specific immunolabel with antiviral glycoprotein antibodies or with conventional lectin label (wheat germ agglutinin) were used in conjunction with colloidal gold-conjugated protein A or ovomucoid, respectively. In addition, intact infected cells were analyzed with both labeling procedures. Experiment...
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Sindbis virus was used as a probe to examine glycosylation processes in two different species of cultured cells. Parallel studies were carried out analyzing the carbohydrate added to Sindbis glycoprotein E2 when the virus was grown in chicken embryo cells and BHK cells. The Pronase glycopeptides of Sindbis glycoprotein E2 were purified by a combination of ion-exchange and gel filtration chromat...
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ورودعنوان ژورنال:
- Journal of virology
دوره 28 2 شماره
صفحات -
تاریخ انتشار 1978